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LifeSensors is a company driven by innovation. Most of our products and technologies have come out of in-house research and development projects. We strongly believe in disseminating knowledge by publication of our key findings. Over the last ten years, LifeSensors scientists have authored or co-authored a number of publications on these technologies. We invite you to request copies of our papers and publications.
Peroutka III RJ, Orcutt SJ, Strickler JE, Butt TR, SUMO fusion technology for enhanced protein expression and purification in prokaryotes and eukaryotes. , Methods in Molecular Biology: Heterologous gene expression in E. Coli, United Kingdom: Humana Press, 2011: 15-30. [Download PDF]
Loch CM, Eddins MJ, Strickler JE, Protein Microarrays for the Identification of Praja1 E3 Ubiquitin Ligase Substrates , Cell Biochemistry and Biophysics, 2011; 60(1-2):127-135. [Download PDF]
Marblestone JG, Kumar KGS, Eddins MJ, Leach CA, Sterner DE, Mattern MR, and Nicholson B, Novel approach for characterizing ubiquitin E3 ligase function. , Journal of Biomolecular Screening, 2010 Dec; 15(10): 1220-8. [Download PDF]
Yan Y, Orcutt SJ, Strickler JE, The use of SUMO as a fusion system for protein expression and purification. , Chemistry Today, 2009 Nov-Dec; 27(6): 42-7.
Hjerpe R, Aillet F, Lopitz-Otsoa F, Lang V, England P, Rodriguez MS, Efficient protection and isolation of ubiquitylated proteins using tandem ubiquitin-binding entities. , EMBO Reports, 2009 Nov;10(11):1250-8.
Hughes SR, Rich JO, Bischoff KM, Hector RE, Qureshi S, Saha BD, Dien BS, Liu S, Cox EJ, Jackson JS, Sterner DE, Butt TR, LaBaer J, Cotta MA, Automated Yeast Transformation Protocol to Engineer Saccharomyces cerevisiae Strains for Cellulosic Ethanol Production with Open Reading Frames That Express Proteins Binding to Xylose isomerase Identified Using a Robotic Two-Hybrid Screen. , Journal of the Association for Laboratory Automation, 2009 Aug; 14(4): 200-12.
Hughes SR, Hector RE, Rich JO, Qureshi N, Bischoff KM, Dien BS, Saha BC, Liu S, Jackson JS, Jr, Sterner DE, Butt TR, LaBaer J, Cotta MA, Automated yeast mating protocol using open reading frames from Saccharomyces cerevisiae genome to improve yeast strains for cellulosic ethanol production. , Journal of the Association for Laboratory Automation, 2009 Aug; 14(4 ): 190-9.
Hughes SR, Sterner DE, Bischoff KM, Hector RE, Dowd PF, Qureshi N, Bang SS, Grynaviski N, Chakrabarty T, Johnson ET, Dien BS, Mertens JA, Caughey RJ, Liu S, Butt TR, LaBaer J, Cotta MA, Rich JO, Three-Plasmid SUMO Yeast Vector System for Automated High-Level Functional Expression of Value- Added Co-Products in a Saccharomyces cerevisiae Strain Engineered for Xylose Utilization. , Plasmid, 2009 Jan; 61(1): 22-38.
Panavas T, Sanders C, Butt TR, SUMO fusion technology for enhanced protein production in prokaryotic and eukaryotic expression systems. , Methods in Molecular Biology: SUMO Protocols, United Kingdom: Humana Press, 2009: 303-17.
Liu L, Spurrier J, Butt TR, Strickler JE, Enhanced protein expression in the baculovirus/insect cell system using engineered SUMO fusions. , Protein Expression and Purification, 2008 Nov; 62(1): 21-8.
Peroutka RJ, Elshourbagy N, Piech T, Butt, TR, Enhanced protein expression in mammalian cells using engineered SUMO fusions: Secreted phospholipase A2. , Protein Science, 2008 Sep; 17(9): 1586-95.
Hughes SR, Dowd PF, Hector RE, Panavas T, Sterner DE, Qureshi N, Bischoff KM, Bang SS, Mertens JA, Johnson ET, Li XL, Jackson JS, Caughey RJ, Riedmuller SB, Bartolett S, Liu S, Rich JO, Farrelly PJ, Butt TR, Labaer J, Cotta MA, Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a coproduct in an ethanologenic Saccharomyces cerevisiae strain. , J Pept Sci, 2008 Sep; 14(9): 1039-50.
Nicholson B, Leach CA, Goldenberg SJ, Francis DM, Kodrasov MP, Tian X, Shanks J, Sterner DE, Bernal A, Mattern MR, Wilkinson KD, Butt TR, Characterization of Ubiquitin and Ubiquitin-like Protein Isopeptidase Activities. , Protein Science, 2008 Jun; 17(6): 1035-43.
Nicholson B, Marblestone JG, Butt TR, Mattern MR., Deubiquitinating Enzymes as Novel Anticancer Targets. , Future Oncology, 2007 Apr; 3(2): 191-9.
Hughes SR, Dowd PF, Hector RE, Riedmuller SB, Bartolett S, Mertens JA, Qureshi N, Liu S, Bischoff KM, Li XL, Jackson, Jr. JS, Sterner D, Panavas T, Rich JO, Farrelly PJ, Butt T, Cotta MA, High-Throughput Multiplex Library Construction of Synthetic Mutagenized Insecticidal Peptides Using a Fully Automated Plasmid-Based Functional Proteomic Robotic Workcell with an Improved Vacuum Block. , J Assoc Lab Automation, 2007. 12(4): 2002-12.
Arnold JJ, Bernal A, Uche U, Sterner DE, Butt TR, Cameron CE, Mattern MR, SUMO isopeptidase assay based on poliovirus RNA polymerase activity. , Anal Biochem, 2006 Mar 15; 350(2): 214-21.
Marblestone JG, Edavettal SC, Lim Y, Lim P, Zuo X, Butt TR, Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO. , Prot Sci, 2006 Jan; 15(1): 182-9.
Hall JP, Applying Fusion Protein Technology to E. coli. , BioPharm International, 2007.
Hall J, Mattern MR, Butt TR., Vision: Mining the Ubiqitin Pathway. , The Scientist, 2005; 19(23): 42-3.
Butt TR, Edavettal SC, Hall JP, Mattern MR, SUMO fusion technology for difficult-to-express proteins. , Protein Expr Purif, 2005 Sep; 43(1): 1-9.
Zuo X, Li S, Hall J, Mattern MR, Tran H, Shoo J, Tan R, Weiss SR, Butt TR, Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli. , J Struct Funct Gen, 2005; 6(2-3): 103-11.
Zuo X, Mattern MR, Tan R, Li S, Hall J, Sterner DE, Shoo J, Tran H, Lim P, Sarafianos SG, Kazi L, Navas-Martin S, Weiss SR, Butt TR., Expression and purification of SARS coronavirus proteins using SUMO-fusions. , Protein Expr Purif, 2005 Jul; 42(1): 100-10.
Hall J, Production of Difficult-to-Express Proteins. , Genetic Engineering News, 2005; 25(9): 52-53.
Malakhov MP, Mattern MR, Malakhov OA, Drinker M, Weeks SD, Butt TR., SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins. , J Struct Funct Gen, 2004; 5(1-2): 75-86.
